How to Culture SH-SY5Y Cells: A Step-by-Step Protocol
How to Culture SH-SY5Y Cells: A Step-by-Step Protocol
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These cells include different specialized types such as epithelial cells, cup cells, parietal cells, chief cells, and enterocytes. Some associated cell lines used in research study to study digestive system feature and diseases consist of Hepa1-6, also referred to as hepa1-6 or hepa 1-6 cells. Other significant cell lines such as Hep2 cells and SCC7 are also commonly used in digestive system research.
Cells of the respiratory system include ciliated epithelial cells, alveolar cells (type I and type II), and goblet cells. When asked what cells are in the respiratory system or what type of cells are in the respiratory system, these cell types form the core answer. The types of cells in the respiratory system are also studied making use of details cell lines, consisting of Calu 6 cell line, also composed as calu-6 or calu6.
Another important cell type in the human body is the mature red blood cell. Generally known as erythrocytes, mature red blood cells are necessary for transporting oxygen from the lungs to tissues and eliminating carbon dioxide. When a person asks what is a mature red blood cell or refers to mature erythrocytes, they are speaking about these enucleated, biconcave cells.
The NB4 cell line is derived from intense promyelocytic leukemia and is used in leukemia research, while the MOLM 13 cell line, also referred to as molm-13 or molm13, is made use of to study intense myeloid leukemia. MB49 cell line, on the various other hand, is a mouse bladder cancer cell line made use of in urological cancer study. RKO cell line is a human colon carcinoma cell line used in colon cancer studies, and Daudi cell line, derived from Burkitt's lymphoma, is extensively used in immunology study.
Further exploring lab cell lines, HEL 92.1.7 is used in hematology research study and is a human erythroleukemia cell line. JIMT cells, particularly JIMT-1, are utilized in bust cancer research study, specifically HER2-positive cancer researches. KP4, also written as KP 4, is a pancreatic cancer cell line. CT2A cell line is a glioma version used in brain cancer cells research. A498 cell line is a renal cancer version used for kidney cancer studies, while RT 112 or RT112 cell line originates from bladder cancer. SK-BR-3 and AU565 are both breast cancer cells cell lines beneficial in HER2-positive cancer research study. Karpas422 is a B-cell lymphoma cell line, and Monomac is a monocytic cell line made use of in immunological studies. LS513 and SW 1353 cell lines add to colon and chondrosarcoma study, specifically. HSC4 is another cancer cell line utilized in dental squamous cancer studies. THLE-2 is a non-tumorigenic liver cell line used in hepatotoxicity research studies.
Biotechnology companies like Accegen give numerous of these cell lines for research objectives, making cells for sale easily available to research laboratories worldwide. Monkey cells, such as Vero cell line and MA104, are utilized in virology and vaccine advancement. COS7, also referred to as COS7 cell, is a monkey kidney cell line typically made use of in transfection research studies.
Specialized cells such as dopaminergic neurons are vital for studying neurological conditions like Parkinson's disease. BAF3 cells are a murine pro-B cell line utilized in hematopoietic researches.
Stable transfection refers to the combination of international DNA right into the host genome, enabling for long-lasting expression of the transgene. The TN5 transposase is a healthy protein utilized in transposon-based genetics editing and enhancing, promoting the insertion of hereditary product into DNA.
In cell culture methods, certain methods are called for to preserve and propagate cells. SH-SY5Y cell culture protocol lays out steps to grow this human neuroblastoma cell line, frequently utilized in neurobiology.
Assorted terms such as ALL PO and 112/84 might refer to scientific or experimental parameters, with 112/84 possibly indicating a blood stress reading. PFSK, slvl, and ymb-1 may represent particular cell lines or speculative markers, while EBTR may refer to a research study device or reagent.
In general, this thorough summary of numerous cells, cell lines, and related biological devices provides a foundational understanding of mobile biology and its application in clinical study. From the crucial features of mature erythrocytes in oxygen transportation to the role of Calu 6 and A549 cells in respiratory research studies, and the relevance of stable transfection in genetic research study, the research study of cells remains at the center of biological science. Each key phrase stands for a foundation in the large landscape of mobile study, adding to advancements in medicine, rehabs, and our understanding of life at the mobile degree.
Discover sh-sy5y cell culture protocol the intricate roles of specialized cells in the human body's digestive, respiratory, and hematological systems, together with essential cell lines used in biomedical research study to improve our understanding of condition devices and therapy feedbacks.